Part:BBa_K1023003:Experience

Applications of BBa_K1023003

The non-codon optimized versions of the FRET ATP bio-sensor(ATeam), which was kindly given by Imamura lab, have been implemented and proven to work in Aspergillus niger and Escherichia coli at our lab, see figure 1 and 2.

Fig.1 non codon-optimized ATP biosensor expression in E.coli

Fig.2 non codon-optimized ATP biosensor expression in A.niger

iGEM Wageningen_UR 2013 Biosensor page

Ateams have been previously developed and implemented to quantify in vivo ATP levels in HeLa and yeast cells [1], [2] .

Reference:

1. Imamura, H., et al. (2009). "Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators." PNAS 106: 15651-15656.
2. Bermejo, C., et al. (2011). In vivo biochemistry: quantifying ion and metabolite levels in individual cells or cultures of yeast. Biochem. J 438 (1–10).

User Reviews

UNIQ33fe491f84569d5f-partinfo-00000002-QINU UNIQ33fe491f84569d5f-partinfo-00000003-QINU